ABSTRACT
Objective:To investigate the effect of epigallocatechin-3-gallate ( EGCG) on the expression of pro-inflammatory cytokines in murine bone marrow-derived macrophages ( BMDM) induced by IFN-γ/LPS.Methods:The bone marrow cells were isolated from 6-8 weeks C57BL/6 mice,which cultured in RPMI1640 medium with 10%FBS and stimulated with 100 ng/ml M-CSF in vitro,and then exposed to 50 ng/ml IFN-γand 1 μg/ml LPS with various concentrations of EGCG (12.5-50 μmol/L) .The expression of pro-inflammatory factors,IL-1β,TNF-αand iNOS were detected,in murine myeloid macrophages stimulated by IFN-γ/LPS with qRT-PCR and ELISA.Results:IFN-γ/LPS remarkably up-regulated the expression levels of inflammatory cytokines ,IL-1β,TNF-αand iNOS,but EGCG effectively repressed these cytokines expression in IFN-γ/LPS-stimulated murine myeloid macrophages , in dose-dependent manner.Conclusion:EGCG attenuates the pro-inflammatory phenotype of murine myeloid macrophages stimulated with IFN-γ/LPS.